γ-[(Propargyl)-imido]-ATP
Catalogue Number: CLK-T11-1-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | solid |
| Type: | Conjugation Reagent |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
Description
Description: Lee et al.[1] reported a non-radioactive version of in vitro phosphorylation were γ-[(Propargyl)-imido]-ATP (compound 1[1]) has been successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2. The phosphorylated, alkyne-modified protein substrate can subsequently be labeled with azides of biotin or fluorescent dyes via Cu(I)- catalyzed Click-Chemistry. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Additional Text
Solubility
10 mM Tris-HCl pH 7.5
Additional Information
Please note: This compound contains a phosphoramide linkage which is hydrolyzed at pH
Application Notes
in vitro phosphorylation of recombinant proteins[1]
Short Description
Adenosine-5'-[γ-(propargyl)-imido]triphosphate, Sodium salt
Smile String
c1(c2c(ncn1)n(cn2)[C@H]1[C@H](O)[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(NCC#C)O)N
CLK-T11-1 MSDS
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