Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein (COV-VM4FOB)
Catalogue Number: COV-VM4FOB-KAT
COV-VM4FOB Image
COV-VM4FOB Image
COV-VM4FOB Image
Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
COV-VM4FOB Image
Immobilized Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 136.70 kDa. Due to glycosylation, the protein migrates to 140-180 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Spike Proteins |
| Alias: | S protein; Spike glycoprotein; S glycoprotein; COVID-19 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Additional Text
Protein Tag
HIS-AviTag™
Accession Number
QHO60594.1
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human ACE2, hFc Tag at 0.5Oeg/ml (100Oel/well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag with the EC50 of 0.91ug/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
Short Description
Recombinant Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F) trimer design. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
COV-VM4FOB Datasheet
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DELIVERY
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