Biotinylated SARS-COV-2 Spike S1 Protein (COV-VM4S1B)
Catalogue Number: COV-VM4S1B-KAT
COV-VM4S1B Image
COV-VM4S1B Image
COV-VM4S1B Image
COV-VM4S1B Image
The purity of Biotinylated SARS-COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC.
COV-VM4S1B Image
Biotinylated SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
COV-VM4S1B Image
Immobilized Biotinylated SARS-COV-2 Spike S1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Spike Proteins |
| Alias: | S1 protein; S glycoprotein Subunit1|S1 protein; Spike protein S1; Spike |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Additional Text
Protein Tag
HIS-AviTag™
Accession Number
QHD43416.1
Short Description
Recombinant Biotinylated SARS-COV-2 Spike S1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln14-Arg683.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Biotinylated SARS-COV-2 Spike S1, Hisi Tag at 1Oeg/ml (100Oel/well) on the streptavidin precoated plate (5Oeg/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 25.5ng/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
COV-VM4S1B Datasheet
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