AgarSqueezer
Catalogue Number: CRI-SOF-1-IDY
| Manufacturer: | Idylle Labs |
| Type: | Tissue culture ware |
| Shipping Condition: | RT |
| Storage Condition: | RT |
| Unit(s): | each |
Description
Description: AgarSqueezer is a device designed to study cell response to short and long term mechanical confinement within a physiological rigidity range. The compression height can be choosen among according to the wafer sizes: 2.5µm, 5µm,30µm, 100µm.
Physiological rigidity
Contrary to PDMS, the mechanical properties of agarose reproduce stiffness of the in vivo microenvironment (1-150 kPa).
Long-term confinement
As the porous nature of agarose facilitates nutrient and oxygen diffusion, the AgarSqueezer provides a safe environment for long-term cell culture. Keep your cells confined for up to 10 days!
Flexibility
By modulating confinement level, matrix stiffness & composition or coating with ECM proteins, AgarSqueezer leaves you plenty of options to reproduce environmental cues.
Applications
Agarsqueezer has been successfully used to confine adherent and non-adherent cells, including:
> Human: primary T-lymphocytes, TF1 & ML2 leukemic cells, HS27A fibroblasts, MCF10A breast cells, MDA-MB-231 breast cancer cells, U-2 OS osteosarcoma cells, PC-3 & DU 145 prostate cancer cells, HT29 & HCT116 colorectal adenocarcinoma cells and HT1080 fibrosarcoma cells
> Murine: megakaryocytes, osteocyte-like cells MLO-Y4, primary muscle cells & primary dendritic cells
> Plant: cells from Arabidopsis roots
> 3D cell cultures: mice gastruloïds
Compatible assays:
> Live imaging*: cell viability, proliferation, migration, morphology, nuclear deformability, cytoskeleton reorganization, cell tracking, etc
> Standard molecular analysis (immunostaining, western blot, qPCR, flow cytometry, etc): changes in gene/protein expression, proliferation, morphological analyses, etc
*Cells can be added with fluorescent probes or stimulated with drugs during confinement.
Imaging modes:
Inverted microscopes equipped with a standard adjustable petri dish holder, with any imaging mode: phase-contrast, epifluorescence, confocal, etc.
Kit contents
1x Agarsqueezer compression device
1x 16G flat cut needle to make holes in the agar gel, facilitating diffusion of culture medium or drugs during the experiment
1x 20G flat cut needle (same as above)
(Optional) 1x device holder (102.5 mm x 143.5 mm), to place and image up to 2 AgarSqueezers in live imaging chambers (Okolab or equivalent).
In addition to basic kit contents, we recommend using a silicium wafer to mold your confining pillars in agarose. We provide four different wafers to mold pillars of various heights.
Additional Text
Short Description
an agarose-based cell confiner
Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations