6*His Monoclonal Antibody
Catalogue Number: CSB-MA000011M0M-CSB
CSB-MA000011M0M Image
CSB-MA000011M0M Image
CSB-MA000011M0M Image
Western Blot Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng All lanes: HIS antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa
CSB-MA000011M0M Image
Western Blot Positive WB detected in: HIS fusion protein 2 at 20ng, 10ng, 5ng, 2.5ng All lanes: HIS antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Ph: | 7.4 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 3G5H8 |
| Isotype: | IgG2a |
| Immunogen: | HHHHHH(6x His) synthetic peptide conjugate to KLH |
| Application: | ELISA, WB |
Description
Description: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
Additional Text
Purification
Protein A purified
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Monoclonal
CSB-MA000011M0m Datasheet
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