Myc tag Monoclonal antibody
Catalogue Number: CSB-MA000041M0M-CSB
CSB-MA000041M0M Image
CSB-MA000041M0M Image
CSB-MA000041M0M Image
CSB-MA000041M0M Image
CSB-MA000041M0M Image
CSB-MA000041M0M Image
CSB-MA000041M0M Image
WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6µg/ml Lane 1: Recombinant Myc-tagged fusion protein at 50ng Lane 2: Recombinant Myc-tagged fusion protein at 25ng Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 50 kd Observed band size: 50 kd
CSB-MA000041M0M Image
Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
CSB-MA000041M0M Image
Immunoprecipitating MYC-tag with transfected HEK293 Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg) Lane 3: Transfected HEK293 whole cell lysate (10µg)
CSB-MA000041M0M Image
WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6µg/ml Lane 1: Recombinant Myc-tagged fusion protein at 50ng Lane 2: Recombinant Myc-tagged fusion protein at 25ng Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 50 kd Observed band size: 50 kd
CSB-MA000041M0M Image
Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
CSB-MA000041M0M Image
Immunoprecipitating MYC-tag with transfected HEK293 Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg) Lane 3: Transfected HEK293 whole cell lysate (10µg)
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Ph: | 7.4 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Mouse |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | EQKLISEEDL (Myc) synthetic peptide conjugate to KLH |
| Application: | ELISA, IF, IP, WB |
Description
Description: Myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein. It can help to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. Myc Tag also can be used to isolate protein complexes with multiple subunits. The Myc Tag Antibody is produced by the conjugation of a synthetic Myc tag peptide to KLH. Myc Tag Antibody can be used in various immunoassays, such as ELISA, Western blotting, immunoprecipitation, immunofluorescence, and more.
Additional Text
Purification
Protein G purified
Antibody Clonality
Monoclonal
CSB-MA000041M0m Datasheet
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