E-Tag Monoclonal Antibody
Catalogue Number: CSB-MA000151M0M-CSB
CSB-MA000151M0M Image
CSB-MA000151M0M Image
CSB-MA000151M0M Image
CSB-MA000151M0M Image
CSB-MA000151M0M Image
CSB-MA000151M0M Image
Western Blot Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng All lanes: E-Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
CSB-MA000151M0M Image
Western Blot Positive WB detected in: E-tagged fusion protein All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
CSB-MA000151M0M Image
Immunoprecipitating E-Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg) Lane 3: 293F transfected whole cell lysate (20µg)
CSB-MA000151M0M Image
ELISA E-Tag antibody in E-tagged fusion protein and Control protein(no E-tag) Antigen at dilution of 1ug/ml E-Tag antibody at (0-0.15ug) Goat polyclonal to Mouse IgG at 1/50000 dilution
CSB-MA000151M0M Image
Western Blot Positive WB detected in: 1-3 lanes: E-tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag All lanes: E-Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 30, 68, 55, 67-157kDa Observed band size: 30, 68, 55, 67-157kDa
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 11H12B3 |
| Isotype: | IgG1 |
| Immunogen: | GAPVPYPDPLEPRC synthetic peptide conjugate to KLH |
| Application: | ELISA, IP, WB |
Additional Text
Purification
Protein G purified
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Monoclonal
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations