V5-Tag Monoclonal Antibody

Catalogue Number: CSB-MA000161M1M-CSB

CSB-MA000161M1M Image

Western Blot Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, 5, 6 at 50ng All lanes: V5 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 31.9, 20.4, 55, 24.2, 53.4, 30 KDa Observed band size: 32, 21, 55, 28, 58, 33 KDa Exposure time: 5min

CSB-MA000161M1M Image

Western Blot Positive WB detected in: 50ng V5-tagged fusion protein V5 Tag antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 KDa Exposure time: 5min

CSB-MA000161M1M Image

Western Blot Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:2000 Lane 1: Recombinant V5-tagged fusion protein at 25ng Lane 2: Recombinant V5-tagged fusion protein at 12.5ng Lane 3: Recombinant V5-tagged fusion protein at 6.25ng Lane 4: Recombinant V5-tagged fusion protein at 3.125ng Lane 5: Recombinant V5-tagged fusion protein at 1.56ng Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 32 kd Observed band size: 32 kd Exposure time: 5min

CSB-MA000161M1M Image

Immunofluorescence staining of transfected HEK293F cells with CSB-MA000161M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L).

CSB-MA000161M1M Image

Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate Lane 1: Mouse control IgG2a instead of CSB-MA000161M1m in Transfected HEK293 cells whole cell lysate Lane 2: CSB-MA000161M1m (2.5µl) + Transfected HEK293F cells whole cell lysate (500µg) Lane 3: Transfected HEK293F cells whole cell lysate (20µg) For western blotting, the blot was detected with CSB-MA000161M1m at 1:3000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000

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Manufacturer:	Cusabio Biotech
Preservative:	0.03% ProClin™ 300
Physical state:	Liquid
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 50 ul
Host name:	Mouse
Clone:	9E8B7
Isotype:	IgG2a
Immunogen:	Synthetic Peptide
Application:	ELISA, IF, IP, WB