CDH1 Monoclonal Antibody
Catalogue Number: CSB-MA005034A0M-CSB
CSB-MA005034A0M Image
CSB-MA005034A0M Image
CSB-MA005034A0M Image
CSB-MA005034A0M Image
CSB-MA005034A0M Image
CSB-MA005034A0M Image
Western Blot Positive WB detected in: Caco205 whole cell lysate All lanes: CDH1 antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 98, 91 kDa Observed band size: 110, 120 kDa
CSB-MA005034A0M Image
Immunofluorescence staining of Hela cells with CSB-MA005034A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
CSB-MA005034A0M Image
Immunofluorescence staining of MCF-7 cells with CSB-MA005034A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
CSB-MA005034A0M Image
Immunofluorescence staining of SW620 cells with CSB-MA005034A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
CSB-MA005034A0M Image
Overlay histogram showing MCF-7 cells stained with CSB-MA005034A0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Cadherin-1 (CAM 120/80) (Epithelial cadherin) (E-cadherin) (Uvomorulin) (CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3], CDH1, CDHE UVO |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 9H2D3 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant Human Cadherin-1 protein (155-707AA) |
| Application: | ELISA, FC, IF, WB |
Description
Description: Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Additional Text
Gene Name
CDH1
Gene ID
999
Uniprot ID
P12830
Purification
Protein G purified
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Monoclonal
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations