ENO1 Monoclonal Antibody

Catalogue Number: CSB-MA007670A0M-CSB

CSB-MA007670A0M Image

Western Blot Positive WB detected in: K562 whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain tissue, Mouse Brain tissue, Rabbit Skeletal Muscle tissue, Rat Kidney tissue, Rabbit Kidney tissue All lanes ENO1 antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 0.261ug/ml Predicted band size: 47 KDa Observed band size: 47 KDa Exposure time: 1min

CSB-MA007670A0M Image

Western Blot Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate All lanes ENO1 antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 0.261ug/ml Predicted band size: 47 KDa Observed band size: 47 KDa Exposure time: 10s

CSB-MA007670A0M Image

Western Blot Positive WB detected in: HepG2 whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg All lanes: ENO1 antibody at 1:5000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 47 kDa Observed band size: 47 KDa Exposure time: 10s

CSB-MA007670A0M Image

Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: ENO1 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 47 kDa Observed band size: 47 KDa Exposure time: 10s

CSB-MA007670A0M Image

IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human liver cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-MA007670A0M Image

IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human colon cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-MA007670A0M Image

IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human pancreas tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-MA007670A0M Image

Immunofluorescence staining of MCF-7 cells with CSB-MA007670A0m at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-MA007670A0M Image

Immunofluorescence staining of Hela cells with CSB-MA007670A0m at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-MA007670A0M Image

Immunoprecipitating ENO1 in HepG2 whole cell lysate Lane 1: Mouse control IgG (1µg) instead of CSB-MA007670A0m in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: CSB-MA007670A1m (2µl) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)

CSB-MA007670A0M Image

Overlay histogram showing MCF-7 cells stained with CSB-MA007670A0m (red line) at 1:260. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

CSB-MA007670A0M Image

Overlay histogram showing Hela cells stained with CSB-MA007670A0m (red line) at 1:260. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

CSB-MA007670A0M Image

Immunofluorescence staining of HepG2 cells with CSB-MA007670A0m at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-MA007670A0M Image

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Manufacturer:	Cusabio Biotech
Preservative:	0.03% ProClin™ 300
Physical state:	Liquid
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	Alpha-enolase (2-phospho-D-glycerate hydro-lyase) (C-myc promoter-binding protein) (Enolase 1) (MBP-1) (MPB-1) (Non-neural enolase) ( NNE) (Phosphopyruvate hydratase) (Plasminogen-binding protein), ENO1, ENO1L1, MBPB1, MPB1
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 50 ul
Host name:	Mouse
Clone:	8H9G12
Isotype:	IgG1
Immunogen:	Recombinant Human Alpha-enolase protein (2-434AA)
Application:	ELISA, FC, IF, IP, WB, IHC

Overview

Description

Description: ENO1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.