GFAP Monoclonal Antibody
Catalogue Number: CSB-MA009369A0M-CSB
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Western Blot Positive WB detected in: Mouse brain tissue, Rat brain tissue All lanes: GFAP antibody at 2.7µg/ml Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 50, 51 kDa Observed band size: 50 kDa
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-MA009369A0m at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human glioma using CSB-MA009369A0m at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA009369A0m at dilution of 1:100
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Immunofluorescent analysis of A549 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunofluorescent analysis of SH-SY5Y cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunofluorescent analysis of U251 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunofluorescent analysis of U87 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Overlay histogram showing SH-SY5Y cells stained with CSB-MA009369A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG2b (10µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Ph: | 7.4 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Glial fibrillary acidic protein, GFAP |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 1C91F1 |
| Isotype: | IgG2b |
| Immunogen: | Recombinant Human GFAP protein (292-432AA) |
| Application: | ELISA, FC, IF, WB, IHC |
Description
Description: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Additional Text
Gene Name
GFAP
Gene ID
2670
Purification
Protein G purified
Uniprot ID
P14136
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Monoclonal
CSB-MA009369A0m Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations