LAG3 Monoclonal Antibody
Catalogue Number: CSB-MA012719A0M-CSB
CSB-MA012719A0M Image
CSB-MA012719A0M Image
CSB-MA012719A0M Image
CSB-MA012719A0M Image
CSB-MA012719A0M Image
Western Blot Positive WB detected in: 1 lane: Recombinant proteins with LAG3; 2 lane: 293F whole cell lysate transfected with LAG3 All lanes: LAG3 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 76, 88 KDa Observed band size: 76, 88 KDa Exposure time : 5min
CSB-MA012719A0M Image
IHC image of CSB-MA012719A0m diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
CSB-MA012719A0M Image
IHC image of CSB-MA012719A0m diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
CSB-MA012719A0M Image
Overlay Peak curve showing A549 cells stained with CSB-MA012719A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CD223 antibody; CD223 antigen antibody; FDC protein antibody; LAG-3 antibody; Lag3 antibody; LAG3_HUMAN antibody; Lymphocyte activating 3 antibody; Lymphocyte activation gene 3 antibody; Lymphocyte activation gene 3 protein antibody; Protein FDC antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 8B12E10 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant Human Lymphocyte activation gene 3 protein (29-450AA) |
| Application: | ELISA, FC, WB, IHC |
Additional Text
Gene Name
LAG3
Gene ID
3902
Uniprot ID
P18627
Purification
Protein G purified
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Monoclonal
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations