NANOG Monoclonal Antibody
Catalogue Number: CSB-MA888008A0M-CSB
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CSB-MA888008A0M Image
CSB-MA888008A0M Image
CSB-MA888008A0M Image
CSB-MA888008A0M Image
CSB-MA888008A0M Image
CSB-MA888008A0M Image
CSB-MA888008A0M Image
CSB-MA888008A0M Image
CSB-MA888008A0M Image
Western Blot Positive WB detected in: Rat brain tissue, Mouse brain tissue All lanes: NANOG antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 35, 33 kDa Observed band size: 46, 42 kDa
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Western Blot Positive WB detected in: MCF-7 whole cell lysate, Ntera-2 whole cell lysate, A549 whole cell lysate All lanes: NANOG antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 35, 33 kDa Observed band size: 46, 40 kDa
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Immunocytochemistry analysis of CSB-MA888008A0m diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CSB-MA888008A0M Image
Immunocytochemistry analysis of CSB-MA888008A0m diluted at 1:100 and staining in Ntera-2 cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CSB-MA888008A0M Image
Immunofluorescence staining of Hela cells with CSB-MA888008A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
CSB-MA888008A0M Image
Immunofluorescence staining of Ntera-2 cells with CSB-MA888008A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
CSB-MA888008A0M Image
Overlay histogram showing Hela cells stained with CSB-MA888008A0m (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
CSB-MA888008A0M Image
Overlay histogram showing Ntera-2 cells stained with CSB-MA888008A0m (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
CSB-MA888008A0M Image
Overlay histogram showing MCF-7 cells stained with CSB-MA888008A0m (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog), NANOG |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 8A1D11 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant Human Homeobox protein NANOG protein (1-305AA) |
| Application: | ELISA, FC, ICC, IF, WB |
Description
Description: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Additional Text
Gene Name
NANOG
Gene ID
79923
Purification
Protein G purified
Uniprot ID
Q9H9S0
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Monoclonal
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations