CAST Antibody
Catalogue Number: CSB-PA004561YA01HU-CSB
CSB-PA004561YA01HU Image
CSB-PA004561YA01HU Image
CSB-PA004561YA01HU Image
CSB-PA004561YA01HU Image
CSB-PA004561YA01HU Image
Western Blot Positive WB detected in: HL60 whole cell lysate, Mouse heart tissue All lanes: CAST antibody at 2.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 77, 76, 64, 72, 82, 85, 83, 75, 81 kDa Observed band size: 77 kDa
CSB-PA004561YA01HU Image
IHC image of CSB-PA004561YA01HU diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CSB-PA004561YA01HU Image
IHC image of CSB-PA004561YA01HU diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CSB-PA004561YA01HU Image
Immunofluorescence staining of A549 cells with CSB-PA004561YA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Ph: | 7.4 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | BS 17 antibody; Calpain inhibitor antibody; Calpastatin antibody; Cast antibody; Heart type calpastatin antibody; ICAL_HUMAN antibody; MGC9402 antibody; Sperm BS 17 component antibody; Sperm BS-17 component antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant Human Calpastatin protein (1-667AA) |
| Application: | ELISA, IF, WB, IHC |
Description
Description: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Additional Text
Gene Name
CAST
Gene ID
831
Uniprot ID
P20810
Purification
Protein G purified
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Polyclonal
CSB-PA004561YA01HU Datasheet
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