Mono-methyl-H1F0 (K101) Antibody
Catalogue Number: CSB-PA010087OA101ME1HU-CSB
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CSB-PA010087OA101ME1HU Image
CSB-PA010087OA101ME1HU Image
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Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: H1F0 antibody at 1:50 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21, 20 kDa Observed band size: 21 kDa
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Immunofluorescence staining of HepG2 cells with CSB-PA010087OA101me1HU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunocytochemistry analysis of CSB-PA010087OA101me1HU diluted at 1:5 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H1F0 (CSB-PA010087OA101me1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Ph: | 7.4 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide sequence around site of Mono-methyl-Lys (101) derived from Human Histone H1.0 |
| Application: | ELISA, ICC, IF, ChIP, WB |
Description
Description: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Additional Text
Uniprot ID
P07305
Gene ID
3005
Purification
Affinity Purified
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Polyclonal
Gene Name
H1-0
Specificity
H1 histone family member 0 antibody; H1(0) antibody; H10 antibody; H10_HUMAN antibody; h1f0 antibody; H1FV antibody; Histone H1'' antibody; Histone H1(0) antibody; Histone H1.0 antibody; Histone H10 antibody; Histone H5 antibody; MGC5241 antibody; N-terminally processed antibody
CSB-PA010087OA101me1HU Datasheet
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