HDAC3 Antibody
Catalogue Number: CSB-PA010239LA01HU-CSB
CSB-PA010239LA01HU Image
CSB-PA010239LA01HU Image
CSB-PA010239LA01HU Image
CSB-PA010239LA01HU Image
CSB-PA010239LA01HU Image
CSB-PA010239LA01HU Image
Western blot All lanes: HDAC3 antibody at 2µg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 50 kDa Observed band size: 49 kDa
CSB-PA010239LA01HU Image
Western Blot Positive WB detected in: Mouse heart tissue All lanes: HDAC3 antibody at 2.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49, 50 kDa Observed band size: 49 kDa
CSB-PA010239LA01HU Image
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA010239LA01HU at dilution of 1:100
CSB-PA010239LA01HU Image
Immunofluorescent analysis of Hela cells using CSB-PA010239LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
CSB-PA010239LA01HU Image
Chromatin Immunoprecipitation Hela(1.2*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-HDAC3 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP010239HU) against the P21 promoter.
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Ph: | 7.4 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | HD3 antibody; HDAC 3 antibody; HDAC3 antibody; HDAC3_HUMAN antibody; Histone deacetylase 3 antibody; RPD3 2 antibody; RPD3 antibody; RPD3-2 antibody; SMAP45 antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant Human Histone deacetylase 3 protein (1-428AA) |
| Application: | ELISA, IF, ChIP, WB, IHC |
Description
Description: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
Additional Text
Gene Name
HDAC3
Gene ID
8841
Uniprot ID
O15379
Purification
Protein G purified
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Polyclonal
CSB-PA010239LA01HU Datasheet
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