Phospho-HIST1H3A (T3) Antibody
Catalogue Number: CSB-PA010418OA03PHHU-CSB
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CSB-PA010418OA03PHHU Image
CSB-PA010418OA03PHHU Image
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Western Blot Detected samples: Jurkat whole cell lysate, Hela whole cell lysate; Untreated (-) or treated (+) with Calyculin A and EGF All lanes: HIST1H3A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
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Immunoprecipitating HIST1H3A in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA010418OA03phHU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA010418OA03phHU (5µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
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Chromatin Immunoprecipitation Hela (4*106, treated with 100nM calyculin A for 60min) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (CSB-PA010418OA03phHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide sequence around site of Phospho-Thr (3) derived from Human Histone H3.1 |
| Application: | ELISA, IP, ChIP, WB |
Description
Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Additional Text
Uniprot ID
P68431
Purification
Affinity Purified
Gene ID
8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Polyclonal
Gene Name
H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8
Specificity
H3 histone family member E pseudogene antibody; H3 histone family; member A antibody; H3/A antibody; H31_HUMAN antibody; H3F3 antibody; H3FA antibody; Hist1h3a antibody; HIST1H3B antibody; HIST1H3C antibody; HIST1H3D antibody; HIST1H3E antibody; HIST1H3F antibody; HIST1H3G antibody; HIST1H3H antibody; HIST1H3I antibody; HIST1H3J antibody; HIST3H3 antibody; histone 1; H3a antibody; Histone cluster 1; H3a antibody; Histone H3 3 pseudogene antibody; Histone H3.1 antibody; Histone H3/a antibody; Histone H3/b antibody; Histone H3/c antibody; Histone H3/d antibody; Histone H3/f antibody; Histone H3/h antibody; Histone H3/i antibody; Histone H3/j antibody; Histone H3/k antibody; Histone H3/l antibody
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations