IGHA2 Antibody
Catalogue Number: CSB-PA011112LA01HU-CSB
CSB-PA011112LA01HU Image
CSB-PA011112LA01HU Image
CSB-PA011112LA01HU Image
IHC image of CSB-PA011112LA01HU diluted at 1:300 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CSB-PA011112LA01HU Image
Immunofluorescence staining of HepG2 cells with CSB-PA011112LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Secondary Antibody - Unconjugated |
| Alias: | IGHA2Immunoglobulin heavy constant alpha 2 antibody; Ig alpha-2 chain C region antibody; Ig alpha-2 chain C region BUT antibody; Ig alpha-2 chain C region LAN antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant Human Immunoglobulin heavy constant alpha 2 protein (36-111AA) |
| Application: | ELISA, IF, IHC |
Description
Description: Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed:2241915).
Additional Text
UniProt ID
P01877
Purification
Protein G purified
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Polyclonal
SUPPORT
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PRODUCT
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DELIVERY
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