TP53 Antibody
Catalogue Number: CSB-PA07889A0RB-CSB
CSB-PA07889A0RB Image
CSB-PA07889A0RB Image
CSB-PA07889A0RB Image
CSB-PA07889A0RB Image
CSB-PA07889A0RB Image
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Rat brain tissue, Mouse brain tissue All lanes: TP53 antibody at 2.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa Observed band size: 44 kDa
CSB-PA07889A0RB Image
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA07889A0Rb at dilution of 1:100
CSB-PA07889A0RB Image
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA07889A0Rb at dilution of 1:100
CSB-PA07889A0RB Image
Chromatin Immunoprecipitation Hela (1.2*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-TP53 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP07889HU) against the P21 promoter.
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Ph: | 7.4 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant Human Cellular tumor antigen p53 protein (2-393AA) |
| Application: | ELISA, ChIP, WB, IHC |
Description
Description: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediatedapoptosis.
Additional Text
Gene Name
TP53
Gene ID
7157
Uniprot ID
P04637
Purification
Protein G purified
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Polyclonal
Specificity
Antigen NY-CO-13 antibody; BCC7 antibody; Cellular tumor antigen p53 antibody; FLJ92943 antibody; LFS1 antibody; Mutant tumor protein 53 antibody; p53 antibody; p53 tumor suppressor antibody; P53_HUMAN antibody; Phosphoprotein p53 antibody; Tp53 antibody; Transformation related protein 53 antibody; TRP53 antibody; tumor antigen p55 antibody; Tumor protein 53 antibody; Tumor protein p53 antibody; Tumor suppressor p53 antibody
CSB-PA07889A0Rb Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations