AGO2 Antibody
Catalogue Number: CSB-PA891731LA01HU-CSB
CSB-PA891731LA01HU Image
CSB-PA891731LA01HU Image
CSB-PA891731LA01HU Image
CSB-PA891731LA01HU Image
CSB-PA891731LA01HU Image
CSB-PA891731LA01HU Image
CSB-PA891731LA01HU Image
CSB-PA891731LA01HU Image
CSB-PA891731LA01HU Image
CSB-PA891731LA01HU Image
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA891731LA01HU at dilution of 1:100
CSB-PA891731LA01HU Image
IHC image of CSB-PA891731LA01HU diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CSB-PA891731LA01HU Image
IHC image of CSB-PA891731LA01HU diluted at 1:1200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CSB-PA891731LA01HU Image
Western blot All lanes: Protein argonaute-2 antibody at 2µg/ml Lane 1: A549 whole cell lysate Lane 2: Jurkats whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Raw264.7 whole cell lysate Lane 6: K562 whole cell lysate Lane 7: Mouse liver tissue Lane 8: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 98, 94 kDa Observed band size: 94 kDa
CSB-PA891731LA01HU Image
Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-AgO2 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP891731HU) against the SLC1A5 promoter.
CSB-PA891731LA01HU Image
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA891731LA01HU at dilution of 1:100
CSB-PA891731LA01HU Image
IHC image of CSB-PA891731LA01HU diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CSB-PA891731LA01HU Image
IHC image of CSB-PA891731LA01HU diluted at 1:1200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CSB-PA891731LA01HU Image
Western blot All lanes: Protein argonaute-2 antibody at 2µg/ml Lane 1: A549 whole cell lysate Lane 2: Jurkats whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Raw264.7 whole cell lysate Lane 6: K562 whole cell lysate Lane 7: Mouse liver tissue Lane 8: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 98, 94 kDa Observed band size: 94 kDa
CSB-PA891731LA01HU Image
Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-AgO2 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP891731HU) against the SLC1A5 promoter.
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Ph: | 7.4 |
| Physical state: | Liquid |
| Type: | ChIP Validated Antibodies |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant Human Protein argonaute-2 protein (517-818AA) |
| Application: | ELISA, ChIP, WB, IHC |
Description
Description: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The minimal RISC appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3\' untranslated region (3\'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Additional Text
Uniprot ID
Q9UKV8
Gene Name
Ago2
Gene ID
27161
Purification
Protein G purified
Antibody Clonality
Polyclonal
Specificity
Ago 2 antibody; AGO2_HUMAN antibody; Argonaute 2 antibody; argonaute 2; RISC catalytic component antibody; Argonaute RISC catalytic component 2 antibody; Argonaute2 antibody; CTA-204B4.6 antibody; dAgo2 antibody; eIF 2C 2 antibody; eIF-2C 2 antibody; eIF2C 2 antibody; Eif2c2 antibody; Eukaryotic translation initiation factor 2C 2 antibody; Eukaryotic translation initiation factor 2C subunit 2 antibody; hAgo2 antibody; MGC3183 antibody; PAZ Piwi domain protein antibody; PPD antibody; Protein argonaute-2 antibody; Protein slicer antibody; Q10 antibody; Slicer protein antibody
CSB-PA891731LA01HU Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations