ACTA1 Recombinant Monoclonal Antibody
Catalogue Number: CSB-RA001205A0HU-CSB
CSB-RA001205A0HU Image
CSB-RA001205A0HU Image
CSB-RA001205A0HU Image
CSB-RA001205A0HU Image
CSB-RA001205A0HU Image
IHC image of CSB-RA001205A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CSB-RA001205A0HU Image
Immunofluorescence staining of HepG2 cells with CSB-RA001205A0HU at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
CSB-RA001205A0HU Image
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Raw264.7 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat heart tissue All lanes: Actin antibody at 0.95µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa
CSB-RA001205A0HU Image
Overlay histogram showing Hela cells stained with CSB-RA001205A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.02% Sodium azide |
| Ph: | 7.4 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | |
| Clone: | 25E3 |
| Isotype: | IgG |
| Immunogen: | A synthesized peptide |
| Application: | ELISA, FC, IF, WB, IHC |
Description
Description: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Additional Text
Gene ID
58
Gene Name
ACTA1
Uniprot ID
P68133
Purification
Affinity Purified
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Recombinant Monoclonal
CSB-RA001205A0HU Datasheet
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