BCL2 Recombinant Monoclonal Antibody
Catalogue Number: CSB-RA002611A0HU-CSB
CSB-RA002611A0HU Image
CSB-RA002611A0HU Image
CSB-RA002611A0HU Image
CSB-RA002611A0HU Image
CSB-RA002611A0HU Image
IHC image of CSB-RA002611A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CSB-RA002611A0HU Image
IHC image of CSB-RA002611A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CSB-RA002611A0HU Image
Overlay histogram showing Jurkat cells stained with CSB-RA002611A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
CSB-RA002611A0HU Image
Western Blot Positive WB detected in:HepG2 whole cell lysate,Jurkat whole cell lysate,MCF-7 whole cell lysate All lanes:BCL2 antibody at 1µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.02% Sodium azide |
| Ph: | 7.4 |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | Apoptosis regulator Bcl-2, BCL2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | |
| Clone: | 2C6 |
| Isotype: | IgG |
| Immunogen: | A synthesized peptide |
| Application: | ELISA, FC, WB, IHC |
Description
Description: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Additional Text
Gene Name
BCL2
Gene ID
596
Uniprot ID
P10415
Purification
Affinity Purified
Storage Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antibody Clonality
Recombinant Monoclonal
CSB-RA002611A0HU Datasheet
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