Biotinylated Human GM-CSF R alpha Protein (CSF-HM42RB)
Catalogue Number: CSF-HM42RB-KAT
CSF-HM42RB Image
CSF-HM42RB Image
CSF-HM42RB Image
CSF-HM42RB Image
CSF-HM42RB Image
Biotinylated Human GM-CSF R alpha, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human GM-CSF, No Tag with an affinity constant of 1.68 nM as determined in SPR assay (Biacore T200).
CSF-HM42RB Image
The purity of Biotinylated Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.
CSF-HM42RB Image
Biotinylated Human GM-CSF R alpha on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
CSF-HM42RB Image
Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 37.7 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.
Additional Text
Protein Tag
HIS-AviTag™
Uniprot ID
P15509-1
Short Description
Recombinant Biotinylated Human GM-CSF R alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu23-Gly320.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 2Oeg/ml (100Oel/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.3ng/ml determined by ELISA. The affinity constant of 1.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
CSF-HM42RB Datasheet
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