"N" SARS-CoV-2 Nucleocapsid mRNA (20 µg, Transfection-ready)

Catalogue Number: CVD19-425A-1-SBI

Manufacturer:System Biosciences
Type:mRNA
Shipping Condition:Dry Ice
Unit(s): 20 ug

Description

Description: Transiently express the SARS-CoV-2 nucleocapsid protein (N protein) in the cells of your choice with this ready-to-transfect mRNA.Speed your studies with ready-to-transfect mRNATransiently express the native (not codon optimized) SARS-CoV-2 nucleocapsid proteinStable SARS-CoV-2 nucleocapsid protein expression is also available as a stable cell line, pre-packaged transduction-ready lentivirus, and ready-to-package lentivectorSupporting Data“N” SARS-CoV-2 Nucleocapsid mRNA is of high-qualityThe high quality of the “N” SARS-CoV-2 Nucleocapsid mRNA can be seen in Figure 1, which shows that the in vitro transcribed mRNA migrates as a single species on a 1.5% denaturing agarose gel.Figure 1. The “N” SARS-CoV-2 mRNA is a high-quality mRNA that migrates as a single species.“N” SARS-CoV-2 Nucleocapsid mRNA is well expressed in HEK293T cellsTo demonstrate the robust expression of the “N” SARS-CoV-2 Nucleocapsid mRNA, we transfected 1 µg into HEK293T cells and isolated total protein after two days. We analyzed 15 µg of total protein via western blot, probing for both the nucleocapsid protein and β-actin. The high expression of nucleocapsid protein can be seen in Figure 2. Figure 2. The “N” SARS-CoV-2 Nucleocapsid mRNA is well expressed in HEK293T cells.