Anti-Mouse DR5 (CD262) (Clone MD5-1) - Purified in vivo GOLD™ Functional Grade

Catalogue Number: D232-LET

Manufacturer:	Leinco Technologies, Inc
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	1 mg, 100 mg, 25 mg, 5 mg, 50 mg
Host name:
Clone:	MD5-1
Isotype:
Immunogen:
Application:	FC, IP, WB, FA, Agon

Overview

Description

Description: DR5 is a member of the TNF-related apoptosis-inducing ligand (TRAIL) receptor family and acts as an agonist receptor that transmits death signals, it is also a transcriptional target of the known oncogene p531,2,3. Additionally, DR5 negatively regulates innate immune responses in dendritic cells4. DR5 is expressed in solid tumors and hematological malignancies in both mouse and humans1, where it induces apoptosis via its functional cytoplasmic death domains2. DR5 initiates apoptosis signals when its ligand, TRAIL, or an agonist monoclonal antibody, e.g., MD5-1, triggers a functional trimer configuration of its transmembrane helices and cytosolic domains1. Apoptosis is induced in many types of transformed cells but not in normal cells5. Since TRAIL death receptors are elevated in a wide range of solid tumors, they are being investigated for the treatment of cancer1,2. MD5-1 was generated by immunizing an Armenian hamster with mouse DR5-Ig fusion protein5. Resulting splenocytes were fused with P3U1 mouse myeloma cells and screened for reactivity to mouse DR5-transfected BHK cells by flow cytometry. MD5-1 reacts with all TRAIL-sensitive tumor cells, including 4T1 mammary carcinoma and R331 renal carcinoma. MD5-1 acts as a death-inducing agonist when cross-linked by streptavidin, anti-hamster Ig monoclonal antibody, or FcR. Cytotoxic activity is completely abrogated by pan-caspase inhibitor z-VAD-fmk.

Additional Text

Concentration

5 mg/ml

Purification

Purified

Antibody Clonality

Monoclonal

Storage Note

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Price

£88.00

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