Dinitrophenol (DNP) (MaxLight 490)
Catalogue Number: D8060-04-ML490-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rat |
| Clone: | 3G135 |
| Isotype: | IgE |
| Immunogen: | Synthetic Dinitrophenol (DNP) |
| Application: | IHC, FLISA |
Description
Description: MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Asthma is a chronic lung disease characterized by airway hyper-responsiveness (AHR) to allergens, airway edema, and increased mucus secretion. Increased levels of circulating IgE and IgG1 antibody and a propensity to allergic responses, atopy, are associated with the development of asthma. Animal models of AHR, where control of the timing of exposure to the initiating antigen, the use of a defined allergen trigger, and genetic manipulation are likely to enhance the understanding of AHR. Dinitrophenol (DNP) has long been employed as a model immunogen as a single antigen to reduce the complexity of modeling AHR. One of the primary aspects of the immune response is the interaction of antigens with lymphocytes to induce the formation of antibodies, that in turn makes the antigen harmless. Much of our current understanding of the antibody response to antigens has been derived by using the antibody-hapten model. One known model uses the dinitrophenyl (DNP) group. Immunization of many mammalian species with DNP-protein conjugates results in production of antibodies specific for DNP and the aa side chains to which it is attached. DNP conjugates (DNP-KLH or DNP-BSA) are useful for the production of antibodies specific for DNP and hemocyanin or BSA. DNP immunization produced a significant variation in the amount and antibody class (IgGs, IgA, IgE, IgM) among strains, and under various experimental conditions. DNP preparations (purity and supplier), routes (intramuscular, intravenous, polymerized, etc), frequency of exposure (single injections, multiple etc) may induce a defined class of antibody and its level may vary as well. DNP-induced antibody production has been used to assess the immune status of normal and immune compromised animals.
Additional Text
Specificity
Recognizes Dinitrophenol.
Purification
Purified
Antibody Clonality
Monoclonal
D8060-04-ML490 Datasheet
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