DMPO Nitrone Adduct (FITC)
Catalogue Number: D8075-94C-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2, 0.1% BSA. Labeled with fluorescein isothiocyanate (FITC). |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 12F1 (N1664A) |
| Isotype: | IgG1 |
| Immunogen: | 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin. |
| Application: | ICC, IF, IHC |
Description
Description: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6). Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals (1). DMPO adducts can be generated with protein and DNA radicals (7).
Additional Text
Caution
FOR RESEARCH USE ONLY
Specificity
Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not react with non-adducted proteins or DNA.
Purification
Protein G purified
Antibody Clonality
Monoclonal
D8075-94C Datasheet
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