Biotinylated Human DNAM-1/CD226 Protein (DAM-HM101B)
Catalogue Number: DAM-HM101B-KAT
DAM-HM101B Image
DAM-HM101B Image
DAM-HM101B Image
DAM-HM101B Image
DAM-HM101B Image
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human DNAM-1, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay (Biacore T200).
DAM-HM101B Image
The purity of Biotinylated Human DNAM-1 is greater than 95% as determined by SEC-HPLC.
DAM-HM101B Image
Biotinylated Human DNAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
DAM-HM101B Image
Immobilized Biotinylated Human DNAM-1, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | CD226 molecule; CD226; DNAM1; PTA1; TLiSA1 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
Additional Text
Gene ID
10666
Gene Name
CD226
Uniprot ID
Q15762
Protein Tag
HIS-AviTag™
Short Description
Recombinant Biotinylated Human DNAM-1/CD226 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu19--Asn247.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
The affinity constant of 264nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
DAM-HM101B Datasheet
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