Anti-CD3 e (Ms,Hu,Mo,Ho,Bo,Sw,Rb,Dg,Ca,Mm,Ck,Bi,Fr) from Rat (HH3E) for FFPE tissues
Catalogue Number: DIA-303-DIA
| Manufacturer: | Dianova |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Lyophilized |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;Cd3e;CD3E;T3E |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ul |
| Host name: | Rat |
| Clone: | HH3E |
| Isotype: | IgG1, kappa |
| Immunogen: | Synthetic peptide from cytoplasmic epitope of CD3: ERPPPVPNPDYEPC |
| Application: | ICC, IHC-P, IHC |
Description
Description: Antibody clone HH3E has been validated specifically for the detection of murine CD3 in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). It detects a conserved epitope on the CD3 epsilon chain in a broad variety of species. CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. Cluster of differentiation 3 (CD3) is composed of four distinct polypeptide chains CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta, that form a multimeric protein complex. The CD3 complex associates non-covalently with the T cell receptor (TCR) and serves as a T cell co-receptor. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. During T cell maturation the expression of CD3 migrates from the cytoplasm of pro-thymocytes to the cell-membrane of thymocytes. The specific appearance at all stages of T cell development make CD3 an ideal marker for normal T cells and T cell neoplasms (lymphomas, leukemias). Moreover, CD3 is a useful immunohistochemical marker for T cells in tissue sections. In a clinical setting in humans, CD3 serves as an important T cell marker for the classification of malignant lymphomas and leukemias. It can also be used to detect T cells in coeliac disease, lymphocytic and collagenous colitis. An anti-CD3 epsilon anti-body (Okt3) has been clinically approved for the induction of immunosuppression in organ transplantation. In animal studies anti-CD3 antibodies can induce tolerance to allografts.
Additional Text
Purification
Purified
Gene ID
12501; 916
Uniprot ID
P07766, P22646
Antibody Clonality
Monoclonal
Gene Name
Cd3e, CD3E
DIA-303 Reference
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