Biotinylated Human DKK1 Protein (DKK-HM401B)
Catalogue Number: DKK-HM401B-KAT
DKK-HM401B Image
DKK-HM401B Image
DKK-HM401B Image
DKK-HM401B Image
DKK-HM401B Image
DKK-HM401B Image
Biotinylated Human DKK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
DKK-HM401B Image
Immobilized Biotinylated Human DKK1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-5, hFc Tag with the EC50 of 66.8ng/ml determined by ELISA (QC Test).
DKK-HM401B Image
Immobilized Biotinylated Human DKK1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.34ug/ml determined by ELISA.
DKK-HM401B Image
Immobilized Anti-DKK1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human DKK1, His Tag with the EC50 of 3.1ng/ml determined by ELISA.
DKK-HM401B Image
The purity of Biotinylated Human DKK1 is greater than 90% as determined by SEC-HPLC.
| Manufacturer: | Kactus |
| Shelf Life: | 24 months |
| Molecular Weight: | The protein has a predicted MW of 28.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | Dickkopf-1; Dkk1; SK |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Additional Text
Gene Name
DKK1
Gene ID
22943
Uniprot ID
O94907
Protein Tag
HIS-AviTag™
Short Description
Recombinant Biotinylated Human DKK1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr32-His266.
Storage Note
-20 to -80°C for 24 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Biotinylated Human DKK1, His Tag at 5Oeg/ml (100Oel/well) on the streptavidin precoated plate (5Oeg/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 3.2ug/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
DKK-HM401B Datasheet
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