Flag-Tag Polyclonal Antibody
Catalogue Number: E-AB-40525-ELA
E-AB-40525 Image
E-AB-40525 Image
E-AB-40525 Image
E-AB-40525 Image
E-AB-40525 Image
Western blotting with Anti-FLAG rabbit polyclonal antibody at dilution of 1:1000.Lane1: FLAG tag transfected HEK 293 whole cell lysate, Lane2: HEK 293 whole cell lysate
E-AB-40525 Image
Immunofluorescent analysis of Hela cells transfected with the plasmid overexpressing Flag fusion Protein, using anti-Flag -Tag polyclonal antibody at 1:200 dilution.
E-AB-40525 Image
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-Flag-Tag Polyclonal Antibody at dilution of 1:12000.
E-AB-40525 Image
IP Result of 293F cells transfected with FLAG-Tag fusion protein,using anti-FLAG-Tag rabbit antibody . Lane 1:input, lane 2: rabbit IgG Isotype Control, Lane 3: anti-FLAG-Tag rabbit antibody
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Preservative: | 0.02% Sodium azide |
| Type: | Tag antibodies |
| Alias: | DDDDK epitope tag;DDDDK epitope tag |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin. |
| Application: | IF, IP, WB |
Description
Description: FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This
Additional Text
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Concentration
1 mg/ml
Storage Note
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
E-AB-40525 Protocol
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