His-Tag Monoclonal Antibody
Catalogue Number: E-AB-48003-ELA
E-AB-48003 Image
E-AB-48003 Image
E-AB-48003 Image
E-AB-48003 Image
Western blot of His-Tag fused recombinant protein with Anti-His mouse monoclonal antibody at dilution of 1:1000.
E-AB-48003 Image
IP Result of 293F cells transfected with His-Tag fusion protein,using anti-His-Tag mouse monoclonal antibody . Lane 1:input, Lane 2: anti-His-Tag mouse monoclonal antibody, Lane 3: mouse IgG1 Isotype Control
E-AB-48003 Image
1x10^6 CHO cells Transfected with a His plasmid were stained with 0.2ug Anti-His tag mAb(E-AB-48003) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Preservative: | 0.02% Sodium azide |
| Type: | Tag antibodies |
| Alias: | 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag; Polyhistidine Tag |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | 1A5 |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin. |
| Application: | FC, IP, WB |
Description
Description: The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells. It is one of the simplest and most widely used purification tags in the world.
Additional Text
Purification
Affinity Purified
Antibody Clonality
Monoclonal
Concentration
1 mg/ml
Storage Note
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
E-AB-48003 Protocol
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