Flag-Tag Monoclonal Antibody
Catalogue Number: E-AB-48025-ELA
E-AB-48025 Image
E-AB-48025 Image
E-AB-48025 Image
E-AB-48025 Image
E-AB-48025 Image
Western blotting with anti-FLAG monoclonal antibody at dilution of 1:5000.lane 1: HEK 293 whole cell lysate, Lane 2: FLAG tag transfected HEK 293 cell lysates
E-AB-48025 Image
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-Flag-Tag Monoclonal Antibody at 1:2000 dilution.
E-AB-48025 Image
Lane 1:HEK-293 transfected with DDDDK-tagged (input).Lane 2: Mouse monoclonal IgG2a instead of E-AB-48025 in HEK-293T transfected with DDDDK-tagged.Lane 3: E-AB-48025 IP in HEK-293T transfected with DDDDK-tagged.
E-AB-48025 Image
1x10^6 CHO cells Transfected with a Flag plasmid were stained with 0.2ug Anti-Flag tag mAb(E-AB-48025) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Preservative: | 0.02% Sodium azide |
| Type: | Tag antibodies |
| Alias: | DDDDK epitope tag;DDDDK epitope tag;DYKDDDDK epitope tag |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | 3H9 |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin. |
| Application: | FC, IF, IP, WB |
Description
Description: FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications.
Additional Text
Purification
Affinity Purified
Antibody Clonality
Monoclonal
Concentration
1 mg/ml
Storage Note
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
E-AB-48025 Protocol
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