DNA-RNA Hybrid Monoclonal Antibody[S9.6]
Catalogue Number: E-AB-48028-ELA
E-AB-48028 Image
E-AB-48028 Image
E-AB-48028 Image
E-AB-48028 Image
Immunofluorescentanalysis of (4% PFA) fixed HeLa cells using DNA-RNA hybrid mouse monoclonal antibody [S9.6] at dilution of 1:500.
E-AB-48028 Image
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using anti-DNA-RNA hybrid Mouse Monoclonal Antibody [S9.6] at dilution of 1:500.
E-AB-48028 Image
Chromatin immunoprecipitation analysis of HeLa cells genomic DNA(gDNA) using DNA-RNA hybrid Mouse Monoclonal Antibody [S9.6] at dilution of 1:200.
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Preservative: | 0.02% Sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | DNA-RNA hybrid;RNA/DNA hybrid |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | S9.6 |
| Isotype: | IgG2a |
| Immunogen: | ΦX174 bacteriophage-derived synthetic DNA/RNA |
| Application: | IF, ChIP |
Description
Description: The DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. The S9.6 monoclonal antibody recognizes DNA-RNA hybrids (also known as R-loops) and does not bind to single or double stranded DNA. The antibody has high affinity for DNA?RNA hybrids but also binds RNA-RNA hybrids that are AU-rich. The specificity of the antibody appears to be determined by a combination of sequence and structural dependency since R?loop sequence affects binding affinity.
Additional Text
Antibody Clonality
Monoclonal
Concentration
1 mg/ml
Purification
Protein A/G Purification
Storage Note
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
E-AB-48028 Protocol
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