Recombinant Vimentin Monoclonal Antibody
Catalogue Number: E-AB-81438-ELA
E-AB-81438 Image
E-AB-81438 Image
E-AB-81438 Image
E-AB-81438 Image
Western blot detection of Vimentin in C6,CHO-K1,Hela cell lysates using Vimentin Rabbit mAb(1:1000 diluted).Predicted band size:54kDa.Observed band size:54kDa.
E-AB-81438 Image
Immunohistochemistry of Vimentin in paraffin-embedded Human tonsil using Vimentin Rabbit mAb at dilution 1:100
E-AB-81438 Image
Immunofluorescence of Vimentin (green) in Hela using Vimentin antibody at dilution 1:20, and DAPI(blue)
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Preservative: | 0.01% Sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CTRCT30;Epididymis luminal protein 113;FLJ36605;HEL113;VIM;VIME;Vimentin |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Rabbit |
| Clone: | R02-8C4 |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide of human Vimentin |
| Application: | IF, IHC-P, WB, IHC |
Description
Description: Vimentin,also named as VIM,belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells,especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092),suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Additional Text
Gene Name
VIM
Gene ID
7431
Uniprot ID
P08670
Concentration
300 ug/ml
Purification
Affinity Purified
Antibody Clonality
Monoclonal
Storage Note
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
E-AB-81438 Protocol
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