STRAP Polyclonal Antibody
Catalogue Number: E-AB-92347-ELA
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Western blot analysis of extracts of various cell lines using STRAP Polyclonal Antibody at 1:1000 dilution.
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Immunofluorescence analysis of H9C2 cells using [KO Validated] STRAP Polyclonal Antibody at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of L929 cells using [KO Validated] STRAP Polyclonal Antibody at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using [KO Validated] STRAP Polyclonal Antibody at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug STRAP Polyclonal Antibody.Western blot was performed from the immunoprecipitate using STRAP Polyclonal Antibody at a dilution of 1:1000.
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Preservative: | 0.02% Sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | STRAP; MAWD; PT-WD; UNRIP; serine-threonine kinase receptor-associated protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 120 ul, 200 ul, 60 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein of human STRAP |
| Application: | IF, IP, WB |
Description
Description: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs, the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. STRAP plays a role in the cellular distribution of the SMN complex. Negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein.
Additional Text
Gene Name
STRAP
Gene ID
11171
Uniprot ID
Q9Y3F4
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Concentration
1 mg/ml
Storage Note
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
E-AB-92347 Protocol
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