(KO Validated) PEPCK/PCK2 Polyclonal Antibody
Catalogue Number: E-AB-92385-ELA
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Western blot analysis of various lysates using PEPCK/PCK2 Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts from wild type(WT) and PEPCK/PCK2 knockout (KO) 293T cells using PEPCK/PCK2 Polyclonal Antibody at 1:1000 dilution.
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Immunohistochemistry of paraffin-embedded mouse kidney using PEPCK/PEPCK/PCK2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded rat kidney using PEPCK/PEPCK/PCK2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescence analysis of NIH-3T3 cells using PEPCK/PEPCK/PCK2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using PEPCK/PEPCK/PCK2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of extracts of HepG2 cells using PEPCK/PEPCK/PCK2 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using PEPCK/PEPCK/PCK2 Polyclonal Antibody at a dilution of 1:1000.
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Preservative: | 0.02% Sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | PCK2; PEPCK; PEPCK-M; PEPCK2; phosphoenolpyruvate carboxykinase 2; mitochondrial |
| Shipping Condition: | Blue Ice |
| Unit(s): | 120 ul, 200 ul, 60 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein of human PEPCK/PCK2 |
| Application: | IF, IP, WB, IHC |
Description
Description: This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.
Additional Text
Gene Name
PCK2
Gene ID
5106
Uniprot ID
Q16822
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Concentration
1 mg/ml
Storage Note
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
E-AB-92385 Protocol
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