(KO Validated) CDK4 Polyclonal Antibody
Catalogue Number: E-AB-93172-ELA
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E-AB-93172 Image
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Western blot analysis of extracts from normal (control) and CDK4 knockout (KO) HeLa cells, using CDK4 Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts of various cell lines using CDK4 Polyclonal Antibody at 1:1000 dilution.
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Immunohistochemistry of paraffin-embedded human lung cancer using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
E-AB-93172 Image
Immunohistochemistry of paraffin-embedded mouse lung using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
E-AB-93172 Image
Immunohistochemistry of paraffin-embedded rat testis using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
E-AB-93172 Image
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CDK4 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using CDK4 Polyclonal Antibody at a dilution of 1:1000.
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Preservative: | 0.01% Thimerosal |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CDK4;CMM3;PSK-J3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 120 ul, 200 ul, 60 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide of human CDK4 |
| Application: | IP, WB, IHC |
Description
Description: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Additional Text
Gene Name
CDK4
Gene ID
1019
Uniprot ID
P11802
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Concentration
1 mg/ml
Storage Note
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
E-AB-93172 Protocol
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