(KO Validated) p53 Polyclonal Antibody
Catalogue Number: E-AB-93317-ELA
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Western blot analysis of extracts of 293T cells using p53 Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts from wild type(WT) and p53 knockout (KO) 293T cells using p53 Polyclonal Antibodyat 1:1000 dilution.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] p53 Polyclonal Antibody at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Confocal immunofluorescence analysis of Hela cells using p53 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
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Confocal immunofluorescence analysis of U2OS cells using p53 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug p53 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using p53 Polyclonal Antibody at a dilution of 1:3000.
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Preservative: | 0.01% Thimerosal |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | TP53; BCC7; LFS1; P53; TRP53; tumor protein p53 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 120 ul, 200 ul, 60 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein of human p53 |
| Application: | IF, IP, WB, IHC |
Description
Description: This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Additional Text
Gene Name
TP53
Gene ID
7157
Uniprot ID
P04637
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Concentration
1 mg/ml
Storage Note
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
E-AB-93317 Protocol
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