Inhibition and Production of Superoxide Anionic Colorimetric Assay Kit(WST-1 Method)
Catalogue Number: E-BC-K001-M-ELA
| Manufacturer: | Elabscience |
| Shelf Life: | 6 months |
| Type: | Detection Kit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 48 T, 96 T, 500 assays |
| Range: | |
| Sensitivity: | |
| Sample type: | Cell Culture Supernatant, Cells, Plasma, Serum, Urine |
| Sample size: |
Description
Description: Superoxide anion free radicals are produced through the reaction system of xanthine and xanthine oxidase. WST-1 (a water-soluble tetrazolium salt) can react with the generated superoxide anion to produce water-soluble formazan. When the tested sample contains the superoxide anion free radical inhibitor, it can inhibit the formation of formazan. When the tested sample contains the substance that produces superoxide anion free radical, it can promote the formation of formazan dye. By colorimetric analysis of WST-1 products, the units of activity of inhibition or production of superoxide anion radical in samples can be calculated.
Additional Text
Storage Note
Reagent3:-20°C;Others:2-8°C
E-BC-K001-M Protocol
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