Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit
Catalogue Number: E-BC-K014-ELA
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Type: | Detection Kit |
| Alias: | NUCB2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 96 T |
| Range: | 0.01-3.0 mmol/L |
| Sensitivity: | |
| Sample type: | Cell Culture Supernatant, Cells, Plasma, Serum, Tissue Homogenate |
| Sample size: |
Description
Description: NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.
Additional Text
Detection Method
Colorimetric
Storage Note
This product can be stored at 2~8°C for 12 months with shading light.|6 months
E-BC-K014 MSDS
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