Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit
Catalogue Number: E-BC-K014-ELA
| Manufacturer: | Elabscience |
| Shelf Life: | 6 months |
| Type: | Detection Kit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 96 T |
| Range: | 0.01-3.0 mmol/L |
| Sensitivity: | |
| Sample type: | Cell Culture Supernatant, Cells, Plasma, Serum, Tissue Homogenate |
| Sample size: |
Description
Description: NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.
Additional Text
Storage Note
2-8°C
E-BC-K014 Protocol
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations