Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)
Catalogue Number: E-BC-K020-M-ELA
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Type: | Enzyme Assays |
| Shipping Condition: | Blue Ice |
| Unit(s): | 96 T, 500 assays |
| Range: | 0.2 -14.4 U/mL |
| Sensitivity: | 0.2 U/mL |
| Sample type: | Ascites, Cells, Plasma, Serum, Tissue, Urine |
| Sample size: |
Description
Description: The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.
Additional Text
Storage Note
Reagent3:-20°C;Others:2-8°C
E-BC-K020-M Protocol
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