CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)
Catalogue Number: E-BC-K022-M-ELA
| Manufacturer: | Elabscience |
| Shelf Life: | 6 months |
| Type: | Enzyme Assays |
| Shipping Condition: | Blue Ice |
| Unit(s): | 48 T, 96 T |
| Range: | 1.35-62 U/mL |
| Sensitivity: | 1.35 U/mL |
| Sample type: | Cell Culture Supernatant, Cells, Plasma, Serum, Tissue, Urine |
| Sample size: |
Description
Description: Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.
Additional Text
Storage Note
Reagent4:-20°C;Others:2-8°C
E-BC-K022-M Protocol
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