Glycogen Colorimetric Assay Kit (Liver/Muscle Samples)
Catalogue Number: E-BC-K073-S-ELA
| Manufacturer: | Elabscience |
| Shelf Life: | 6 months |
| Type: | Detection Kit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 assays, 50 assays |
| Range: | 1.80-180 mg/g liver |
| Sensitivity: | 1.80 mg/g liver tiss |
| Sample type: | Tissue |
| Sample size: |
Description
Description: Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen.
Additional Text
Storage Note
2-8°C
E-BC-K073-S Protocol
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