Reactive Oxygen Species (ROS) Fluorometric Assay Kit
Catalogue Number: E-BC-K138-F-ELA
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Type: | Detection Kit |
| Alias: | ROS;DCFH-DA;DCFH;DCF |
| Shipping Condition: | Blue Ice |
| Unit(s): | 96 T |
| Range: | |
| Sensitivity: | |
| Sample type: | Cell Culture, Tissue |
| Sample size: |
Description
Description: DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
Additional Text
Detection Method
Fluorometric method
Storage Note
This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.|6 months
E-BC-K138-F Protocol
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