Sucrose Colorimetric Assay Kit
Catalogue Number: E-BC-K161-S-ELA
| Manufacturer: | Elabscience |
| Shelf Life: | 6 months |
| Type: | Detection Kit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 assays, 50 assays |
| Range: | 0.32-70 µmol/mL |
| Sensitivity: | 0.32 µmol/mL |
| Sample type: | Tissue |
| Sample size: |
Description
Description: Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.
Additional Text
Storage Note
2-8°C
E-BC-K161-S Protocol
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