Sucrose Colorimetric Assay Kit
Catalogue Number: E-BC-K161-S-ELA
| Manufacturer: | Elabscience |
| Shelf Life: | 12 months |
| Type: | Detection Kit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 assays, 50 assays |
| Range: | 0.32-70 µmol/mL |
| Sensitivity: | 0.32 µmol/mL |
| Sample type: | Tissue |
| Sample size: |
Description
Description: Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.
Additional Text
Detection Method
Colorimetric
Storage Note
This product can be stored at 2~8°C for 12 months with shading light.|6 months
E-BC-K161-S Protocol
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