Sodium (Na+) Colorimetric Assay Kit

Catalogue Number: E-BC-K207-S-ELA

Manufacturer:	Elabscience
Shelf Life:	12 months
Type:	Detection Kit
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	200 assays
Range:	80-180 mmol/L
Sensitivity:
Sample type:	Serum
Sample size:

Overview
Datasheets

Description

Description: Application This kit can be used for detecting the concentration of sodium ions in serum samples. Detection principle Production of o-nitrophenol and galactose by o-nitrophenol-β-D-galactoside(ONPG) catalyzed by sodium dependent β-D-galactosidase. The amount of o-nitrophenol is directly proportional to the concentration of sodium ion in the sample. The o-nitrophenol is yellow in alkaline environment. The increase of absorbance is determined at 405nm, and the content of sodium ion is calculated indirectly. Experiment instruments Spectrophotometer (405 nm), Biochemical analyzer (405 nm), Microplate reader(405 nm), Micropipettor Sample requirement Separate the serum as soon as possible after blood collection to avoid hemolysis. Operation steps 1. Detection with Microplate reader: 2. Detection with spectrophotometer: 3. Detection with biochemical analyzer: input the parameters follow the table. Relevant parameters 1. Absorbance of blank: A405 ≤ 0.5, A405 ≤0.4 (optical path = 1 cm) 2. Linear range: 80~180 µmol/L, |R| ≥0.995. 3. Sensitivity: (|A|/min) is between 0.0011 ~ 0.0036 when testing 140 mmol/L samples. 4. Accuracy: relative deviation ≤ 10%. 5. Precision: intra-CV≤ 5.0%, inter- CV ≤ 8%. Notes 1. This kit is for research use only. 2. Please operate strictly according to operation procedures.

E-BC-K207-S Protocol

Price

£96.00

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