Anti-Ebolavirus GP [Clone EBOV-548] - Purified No Carrier Protein

Catalogue Number: E172-LET

Manufacturer:	Leinco Technologies, Inc
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	1 mg, 250 ug
Host name:
Clone:	EBOV-548
Isotype:
Immunogen:
Application:	ELISA, EM, FA, NT

Overview

Description

Description: Ebola virus is a member of the Filoviridae family that causes severe disease in humans with a mortality rate of 25-90%1. Three Ebola species are responsible for lethal outbreaks: Zaire ebolavirus (EBOV), Bundibugyo ebolavirus (BDBV), and Sudan ebolavirus (SUDV). The Ebola virus envelope contains a single surface glycoprotein (GP) which is responsible for viral attachment to the host cell, endosomal entry, and membrane fusion1. GP is composed of two subunits, GP1 and GP2. GP1 has a heavily glycosylated mucin-like domain and a glycan cap. GP2 contains the internal fusion loop, transmembrane domain, and stalk. GP is the major target of neutralizing monoclonal antibody (mAb) and vaccine design against Ebola virus1,2,3,4. EBOV-548 is a pan-EBOV-neutralizing mAb isolated from a survivor of the EBOV 2013-2016 outbreak2. Hybridomas were generated from human peripheral blood mononuclear cells. EBOV GP-reactive memory B cells were labeled with recombinant EBOV GP protein, purified by FACS, bulk expanded on NIH 3T3 cells, bulk fused with MFP-2 myeloma cells, and screened for neutralizing activity against live and/or recombinant EBOV, BDBV, and SUDV GP. EBOV-548 reacts to all three species GPs and neutralizes EBOV and BDBV. EBOV-548 recognizes intact but not cleaved GP. EBOV-548 was identified in a study designed to develop a cooperative two-antibody cocktail with EBOV-5202,4. When paired, EBOV-548 and EBOV-520 have synergistic activity for GP binding and virus neutralization2. This is achieved mechanically. EBOV-548 binds to the glycan cap in a manner that destabilizes the GP trimer, displacing the β17-β18 loop. As a result, the glycan cap is pulled back, facilitating EBOV-520 binding and enhancing neutralization. The cooperative effect is dependent on EBOV-548 concentration. When administered together in vivo, EBOV-548 potentiates protection by EBOV-520 against SUDV, with 50% of mice surviving treatment, while only 10% survive with EBOV-520 alone.

Additional Text

Purification

Purified

Concentration

1 mg/ml

Antibody Clonality

Monoclonal, Recombinant Monoclonal

Storage Note

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Price

£332.00

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