Escherichia coli, J5 LPS (E. coli) (MaxLight 490)
Catalogue Number: E3500-28J-ML490-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 10C141 (2D7/1) |
| Isotype: | IgG2b |
| Immunogen: | E. coli J5 cells |
| Application: | WB, FLISA |
Description
Description: MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
Additional Text
Specificity
Recognizes E. coli J5 LPS. Species crossreactivity: K. pneumoniae, S. sonnei and S. typhimurium.
Purification
Protein A purified
Antibody Clonality
Monoclonal
E3500-28J-ML490 Datasheet
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