FITC-Labeled Human EGFRVIII Protein (EG8-HM154F)
Catalogue Number: EG8-HM154F-KAT
EG8-HM154F Image
EG8-HM154F Image
EG8-HM154F Image
EG8-HM154F Image
EG8-HM154F Image
FITC-Labeled Human EGFRVIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
EG8-HM154F Image
Immobilized FITC-Labeled Human EGFRVIII, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA (QC Test).
EG8-HM154F Image
FACS Analysis of Anti-EGFRVIII CAR Expression. 293T cells were transfected with anti-EGFRVIII-scFv and His tag. Cells were incubated with 5ug/ml FITC-Labeled Human EGFRVIII, His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.
EG8-HM154F Image
The purity of FITC-Labeled Human EGFRVIII is greater than 95% as determined by SEC-HPLC.
| Manufacturer: | Kactus |
| Shelf Life: | 6 months |
| Molecular Weight: | The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result. |
| Physical state: | Liquid |
| Type: | Protein - Conjugated |
| Alias: | ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | Dry Ice |
| Unit(s): | 100 ug, 500 ug |
| Application: | FACS, FC, WB |
Description
Description: The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Additional Text
Protein Tag
HIS-AviTag™
Accession Number
NP_001333870.1
Short Description
Recombinant FITC-Labeled Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser378.
Storage Note
Valid for 6 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Endotoxin
Less than 1 EU per µg by the LAL method.
EG8-HM154F Datasheet
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